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molecular weight marker  (Bio-Rad)


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    Bio-Rad molecular weight marker
    Molecular Weight Marker, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight marker/product/Bio-Rad
    Average 96 stars, based on 1842 article reviews
    molecular weight marker - by Bioz Stars, 2026-03
    96/100 stars

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    Kinetic comparison <t>in</t> <t>SDS-PAGE</t> (12%) of total protein (TP) produced in cultures of E. coli BL21 (DE3) grow up in shake flasks ( A , B ) and bioreactors ( C , D ). RBD-P producing cultures are shown in ( A) and (C ) and RBD in ( B ) and ( D ). In all gels, lanes 1 and 6 correspond to PT before induction. Lanes 2, 3, and 4 (7,8, and 9) show samples taken from cultures at 1, 5, and 10 h after induction, respectively. Lane 5 corresponds to the molecular weight marker (MWM). Lanes 6 to 9 are samples from replica experiments. Recombinant proteins are marked by a black arrow
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    molecular weight marker  (Bio-Rad)
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    Kinetic comparison <t>in</t> <t>SDS-PAGE</t> (12%) of total protein (TP) produced in cultures of E. coli BL21 (DE3) grow up in shake flasks ( A , B ) and bioreactors ( C , D ). RBD-P producing cultures are shown in ( A) and (C ) and RBD in ( B ) and ( D ). In all gels, lanes 1 and 6 correspond to PT before induction. Lanes 2, 3, and 4 (7,8, and 9) show samples taken from cultures at 1, 5, and 10 h after induction, respectively. Lane 5 corresponds to the molecular weight marker (MWM). Lanes 6 to 9 are samples from replica experiments. Recombinant proteins are marked by a black arrow
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    Kinetic comparison in SDS-PAGE (12%) of total protein (TP) produced in cultures of E. coli BL21 (DE3) grow up in shake flasks ( A , B ) and bioreactors ( C , D ). RBD-P producing cultures are shown in ( A) and (C ) and RBD in ( B ) and ( D ). In all gels, lanes 1 and 6 correspond to PT before induction. Lanes 2, 3, and 4 (7,8, and 9) show samples taken from cultures at 1, 5, and 10 h after induction, respectively. Lane 5 corresponds to the molecular weight marker (MWM). Lanes 6 to 9 are samples from replica experiments. Recombinant proteins are marked by a black arrow

    Journal: Microbial Cell Factories

    Article Title: Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19

    doi: 10.1186/s12934-024-02320-5

    Figure Lengend Snippet: Kinetic comparison in SDS-PAGE (12%) of total protein (TP) produced in cultures of E. coli BL21 (DE3) grow up in shake flasks ( A , B ) and bioreactors ( C , D ). RBD-P producing cultures are shown in ( A) and (C ) and RBD in ( B ) and ( D ). In all gels, lanes 1 and 6 correspond to PT before induction. Lanes 2, 3, and 4 (7,8, and 9) show samples taken from cultures at 1, 5, and 10 h after induction, respectively. Lane 5 corresponds to the molecular weight marker (MWM). Lanes 6 to 9 are samples from replica experiments. Recombinant proteins are marked by a black arrow

    Article Snippet: A homemade luminescent strip on top of the PVDF membrane was used to detect immunoreactive proteins placed in the same place as the SDS-PAGE molecular weight marker (ThermoFisher, USA).

    Techniques: Comparison, SDS Page, Produced, Molecular Weight, Marker, Recombinant

    Coomassie blue stained 12% acrylamide gel electrophoresis of the IBs obtained at the end of E. coli BL21 (DE3) cultures producers of RBD-P and RBD. IBs from cultures in shake flasks ( A ); lane 1: molecular weight marker (MWM), lane 2: IBs of RBD-P, and lane 3: IBs of RBD. IBs from two independent bioreactor cultures ( B ); lanes 1 and 4 are IBs from cultures producers of RBD-P, lane 3 is the MWM, and lanes 2 and 5 are IBs from cultures producers of RBD. Immunodetection by Western Blot of purified RBD-P and RBD ( C ) recombinantly produced in E. coli BL21 (DE3) in bioreactor and detected with anti-Spike antibody (Sino Biological 40,591-MM43); lane 1: RBD-P, and lane 2: RBD, and lanes 3 and 4: molecular weight marker (MWM)

    Journal: Microbial Cell Factories

    Article Title: Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19

    doi: 10.1186/s12934-024-02320-5

    Figure Lengend Snippet: Coomassie blue stained 12% acrylamide gel electrophoresis of the IBs obtained at the end of E. coli BL21 (DE3) cultures producers of RBD-P and RBD. IBs from cultures in shake flasks ( A ); lane 1: molecular weight marker (MWM), lane 2: IBs of RBD-P, and lane 3: IBs of RBD. IBs from two independent bioreactor cultures ( B ); lanes 1 and 4 are IBs from cultures producers of RBD-P, lane 3 is the MWM, and lanes 2 and 5 are IBs from cultures producers of RBD. Immunodetection by Western Blot of purified RBD-P and RBD ( C ) recombinantly produced in E. coli BL21 (DE3) in bioreactor and detected with anti-Spike antibody (Sino Biological 40,591-MM43); lane 1: RBD-P, and lane 2: RBD, and lanes 3 and 4: molecular weight marker (MWM)

    Article Snippet: A homemade luminescent strip on top of the PVDF membrane was used to detect immunoreactive proteins placed in the same place as the SDS-PAGE molecular weight marker (ThermoFisher, USA).

    Techniques: Staining, Acrylamide Gel Assay, Electrophoresis, Molecular Weight, Marker, Immunodetection, Western Blot, Purification, Produced